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ENU Mutagenesis
The goal of project III is to
determine the function of genes on mouse Chromosome 11 by saturating the chromosome with
recessive mutations.
The distal 40 cM of mouse Chr 11 exhibits linkage conservation
with human Chromosome 17. We are using the chemical N-ethyl-N-nitrosourea (ENU)
to saturate wild type chromosomes with point mutations. By determining the function of genes on
a mouse chromosome, we can extrapolate to predict function on a human chromosome.
We expect many of the new mutants to represent models of human diseases such as birth defects,
patterning defects, growth and endocrine defects, neurological anomalies, and blood defects.
Because many of the mutations we expect to isolate may be lethal or detrimental to the mice,
we are using a unique approach to isolate mutations.
This approach uses a balancer chromosome that is homozygous lethal and carries a dominant
coat color marker to suppress recombination over a reasonable interval.
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Why use ENU as a mutagen
Balancer chromosomes
Using a deletion scheme to isolate mutations
Using balancer chromosomes to isolate mutations
Phenotype Screen
The Mouse Genome
Project
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Mail: One Baylor Plaza, Houston, Texas 77030
Phone: 713-798-1197
Contact: biliu@bcm.tmc.edu
Last modified: August
21, 2006
